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Journal / Bioengineering / Metanol selvstendig uttrykk av Pichia Pastoris…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Metanol selvstendig uttrykk av Pichia Pastoris ansette de undertrykkelse teknologi
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Metanol selvstendig uttrykk av Pichia Pastoris ansette de undertrykkelse teknologi

Article DOI: 10.3791/58589-v • 05:30 min • January 23rd, 2019
January 23rd, 2019 •
1, 2, 2, 2, 1,2
1bisy e.U., 2Institute of Molecular Biotechnology, Technical University of Graz

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Denne metoden for første gang beskriver pålitelig eksperimentelle prosedyrer for effektiv induserbart metanol-fri rekombinante proteiner produksjon i Pichia pastoris (Komagataella phaffii), ansette karbon kilde regulert arrangører P DCog PDF småskala eksperimenter mens dyrking parametere kan bli overvåket online, og en konstant glyserol feed brukes.

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Bioteknologi problemet 143 Pichia pastoris Komagataella phaffii arrangøren derepression mate kontroll riste flasker dyrking
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