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Journal / Bioengineering / Methanol uafhængige udtryk af Pichia Pastoris…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Methanol uafhængige udtryk af Pichia Pastoris beskæftiger de undertrykkelse teknologier
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Methanol uafhængige udtryk af Pichia Pastoris beskæftiger de undertrykkelse teknologier

Article DOI: 10.3791/58589-v • 05:30 min • January 23rd, 2019
January 23rd, 2019 •
1, 2, 2, 2, 1,2
1bisy e.U., 2Institute of Molecular Biotechnology, Technical University of Graz

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Denne metode for første gang beskrives pålidelige eksperimentelle procedurer for effektiv inducerbar methanol-fri rekombinante protein produktion i Pichia pastoris (Komagataella phaffii), beskæftiger carbon kilde reguleret initiativtagere P DCog PDF i lille skala eksperimenter mens dyrkning parametre kan overvåges online, og en konstant glycerol foder anvendes.

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Bioteknologi spørgsmålet 143 Pichia pastoris Komagataella phaffii projektleder derepression feed control ryste kolber dyrkning
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