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Journal / Neuroscience / Biocytin herstel en 3D-reconstructies van gevulde …
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Biocytin herstel en 3D-reconstructies van gevulde Hippocampal CA2 interneuronen
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Biocytin herstel en 3D-reconstructies van gevulde Hippocampal CA2 interneuronen

Article DOI: 10.3791/58592-v • 11:21 min • November 20th, 2018
November 20th, 2018 •
*1, *1, 1
1Department of Pharmacology, University College London
* These authors contributed equally

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Click here for the English version.

Het protocol hier geschetste beschrijft de immunofluorescentie analyse, biocytin herstel en kwalitatief hoogwaardige reconstructies van hippocampal CA2 interneuronen na de intracellulaire elektrofysiologische opnames in vitro waardoor neuronale karakterisering en uiteindelijk prima neuronale anatomie worden bestudeerd.

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Neurowetenschappen kwestie 141 immunohistochemie immunofluorescentie protocol HRP protocol neuronale reconstructies Neurolucida Camera Lucida neuronale anatomie dendrites axon
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