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Journal / Biochemistry / Structure Solution of the Fluorescent Protein…
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Biochemistry

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JoVE Journal Biochemistry
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
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Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Article DOI: 10.3791/58594-v • 06:42 min • March 19th, 2019
March 19th, 2019 •
*1, *1, 2, 1, 1, 1, 1,3, 1, 1
1European Synchrotron Radiation Facility, Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)
* These authors contributed equally

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Summary
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We present the use of the MeshAndCollect protocol to obtain a complete diffraction data set, for use in subsequent structure determination, composed of partial diffraction data sets collected from many small crystals of the fluorescent protein Cerulean.

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MeshAndCollect Serial Crystallography Rotation Data Collection Small Crystals Structure Solution Position Identification Partial Data Set Merge Ligand Screening Synchrotron Beamline VMX High Photon Flux Small Beam Diameter Fast Reload Detector Extended Information System Protein Crystallography Database Experiment Number Password Shipment Add New SC3 Puck Sample Holders Experimental Hutch Sample Changer Doer Information System
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