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Journal / Biochemistry / Strukturen løsning av fluorescerende Protein…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Strukturen løsning av fluorescerende Protein Cerulean bruker MeshAndCollect
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Strukturen løsning av fluorescerende Protein Cerulean bruker MeshAndCollect

Article DOI: 10.3791/58594-v • 06:42 min • March 19th, 2019
March 19th, 2019 •
*1, *1, 2, 1, 1, 1, 1,3, 1, 1
1European Synchrotron Radiation Facility, Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)
* These authors contributed equally

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Vi presenterer bruken av MeshAndCollect-protokollen for å få et komplett Diffraksjon datasett, for bruk i etterfølgende proteinstrukturer, består av delvis Diffraksjon datasett fra mange små krystaller av fluorescerende protein Cerulean.

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Biokjemi problemet 145 krystall veksten og montering macromolecular Røntgenkrystallografi programvare for beamline (MXCuBE2) informasjon styringssystem for macromolecular krystallografi X-ray eksperimenter (ISPyB) serielle krystallografi MeshAndCollect synchrotron stråling.
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