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Journal / Immunology and Infection / Ensaio de ELISpot otimizado de Interferon-gama às …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Ensaio de ELISpot otimizado de Interferon-gama às respostas de célula T medida no modelo de porquinho da Índia após a vacinação
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Ensaio de ELISpot otimizado de Interferon-gama às respostas de célula T medida no modelo de porquinho da Índia após a vacinação

Article DOI: 10.3791/58595-v • 08:13 min • January 20th, 2019
January 20th, 2019 •
1, 2, 1, 1, 1, 1, 1, 1, 1
1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute

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O desenvolvimento do ensaio ELISpot para cobaia PBMCs interferão-gama permite a caracterização das respostas de células T neste modelo altamente relevantes para o estudo de doenças infecciosas. Temos aplicado o ensaio para medir respostas de células T associadas a intradérmica entrega de vacinas de DNA.

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Imunologia e infecção edição 143 enzima-lig imune local ensaio cobaias linfócitos-T vacinação interferon-gama administração cutânea
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