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Journal / Environment / Tilapia Gölü virüs kullanarak geleneksel RT-PCR…
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Tilapia Gölü virüs kullanarak geleneksel RT-PCR ve SYBR yeşil RT-qPCR
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Tilapia Gölü virüs kullanarak geleneksel RT-PCR ve SYBR yeşil RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

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Bu iletişim kuralı Tilapia Gölü virüs (TiLV) RT-PCR yöntemlerini kullanarak tilapia dokularda tanılar. Tüm yöntemi doku diseksiyon toplam RNA emme, ardından cDNA sentezi ve TiLV geleneksel PCR veya kantitatif PCR dsDNA bir floresan bağlama boya bağlama kullanarak tarafından algılanması için tanımlanır.

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Çevre Bilimleri sayı: 141 Tilapia Gölü virüs tilapia tanı RT-PCR RT-qPCR orthomyxo gibi virüs
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