Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Environment / Påvisning af Tilapia søen Virus ved hjælp af…
JoVE Journal
Environment
Author Produced

This content is Open Access.

JoVE Journal Environment
Påvisning af Tilapia søen Virus ved hjælp af konventionelle RT-PCR og SYBR Green RT-qPCR
Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production
Journal (Environment)
Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production
A Push-pull Protocol to Reduce Colonization of Bird Nest Boxes by Honey Bees
Journal (Environment)
A Push-pull Protocol to Reduce Colonization of Bird Nest Boxes by Honey Bees
 
Click here for the English version

Påvisning af Tilapia søen Virus ved hjælp af konventionelle RT-PCR og SYBR Green RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol diagnoser Tilapia søen Virus (TiLV) i tilapia væv ved hjælp af RT-PCR metoder. Den hel metode er beskrevet af væv dissektion til total RNA udvinding, efterfulgt af cDNA syntese og påvisning af TiLV af enten konventionel PCR eller kvantitativ PCR ved hjælp af dsDNA bindende en fluorescerende bindende farvestof.

Tags

Miljøvidenskab sag 141 Tilapia søen virus tilapia diagnose RT-PCR RT-qPCR orthomyxo-lignende virus
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter