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4D microscopie van gist
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4D microscopie van gist

Article DOI: 10.3791/58618-v • 12:00 min • April 28th, 2019
April 28th, 2019 •
1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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Dit protocol beschrijft de analyse van fluorescently gelabelde intracellulaire compartimenten in ontluikende gist met behulp van Multi-Color 4D (time-lapse 3D) confocale microscopie. De beeldvormings parameters worden gekozen om adequate signalen vast te leggen en foto schade te beperken. Met aangepaste ImageJ-plug-ins kunnen gelabelde structuren worden bijgehouden en kwantitatief worden geanalyseerd.

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Biologie uitgave 146 confocale microscopie 4D-beeldvorming fluorescentie fotobleaching deconvolutie ImageJ gist
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