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April 28, 2019
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Summary
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Questo protocollo descrive l'analisi dei compartimenti intracellulari etichettati fluorescentmente in lievito in erba utilizzando la microscopia confocale 4D (time-lapse 3D). I parametri di imaging vengono scelti per catturare segnali adeguati limitando i fotodanni. I plug-in Custom ImageJ consentono di tenere traccia delle strutture etichettate e di analizzarle quantitativamente.
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Cite this Article
Johnson, N., Glick, B. S. 4D Microscopy of Yeast. J. Vis. Exp. (146), e58618, doi:10.3791/58618 (2019).
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