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Journal / Bioengineering / Måling af kraft-følsom Protein Dynamics i levende …
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JoVE Journal Bioengineering
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Måling af kraft-følsom Protein Dynamics i levende celler ved hjælp af en kombination af fluorescerende teknikker

Article DOI: 10.3791/58619-v • 08:28 min • November 2nd, 2018
November 2nd, 2018 •
1, 1, 1
1Department of Biomedical Engineering, Duke University

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Vi præsenterer her, en protokol til den samtidige brug af Förster resonans energi transfer-baseret spænding sensorer til at måle protein belastning og fluorescens opsving efter photobleaching til at måle protein dynamics muliggør måling af kraft-følsom protein dynamikken i levende celler.

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Bioteknologi sag 141 Mechanobiology mechanotransduction Biofysik FRET-baserede spænding sensorer FRAP kraft-følsom protein dynamics
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