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Journal / Immunology and Infection / Digestione del fegato murino per un'analisi…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Digestione del fegato murino per un'analisi citofluorimetrica delle cellule endoteliali linfatiche
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Digestione del fegato murino per un'analisi citofluorimetrica delle cellule endoteliali linfatiche

Article DOI: 10.3791/58621-v • 08:07 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1,2
1Division of Gastroenterology and Hepatology, Department of Medicine, University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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L'obiettivo del presente protocollo è quello di identificare popolazioni di cellule endoteliali linfatiche all'interno del fegato usando gli indicatori descritti. Utilizziamo collagenasi IV e DNasi e una delicata macinazione del tessuto, combinato con citometria a flusso, per identificare una popolazione distinta di cellule endoteliali linfatiche.

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Immunologia e infezione numero 143 fegato cellule endoteliali linfatiche citometria a flusso le cellule endoteliali sinusoidali epatiche collagenasi IV
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