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Arricchire ed espandere cellule T antigene-specifiche rara con nanoparticelle magnetiche
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Arricchire ed espandere cellule T antigene-specifiche rara con nanoparticelle magnetiche

Article DOI: 10.3791/58640-v • 09:28 min • November 17th, 2018
November 17th, 2018 •
1,2,3,4, 2,4
1Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, 2Institute for Cell Engineering, School of Medicine, Johns Hopkins University, 3Institute for Nanobiotechnology, Johns Hopkins University, 4Department of Pathology, School of Medicine, Johns Hopkins University

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Cellule T antigene-specifiche sono difficili da caratterizzare o utilizzare in terapie grazie alla loro frequenza estremamente bassa. Qui, forniamo un protocollo per sviluppare una particella magnetica che si può associare a cellule T antigene-specifiche per arricchire queste cellule e quindi di espanderli diversi moltiplicati per la caratterizzazione e la terapia.

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Ingegneria numero 141 nanoparticelle arricchimento magnetico artificiale cellule presentanti l'antigene cellule T immunoterapia cancro arricchimento di rara delle cellule espansione a cellula T terapia adottiva T cellulare leggermente rallentata e immunoengineering Bioingegneria
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