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Enriquecer e expandir células raras T antígeno-específicos com nanopartículas magnéticas
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Enriquecer e expandir células raras T antígeno-específicos com nanopartículas magnéticas

Article DOI: 10.3791/58640-v • 09:28 min • November 17th, 2018
November 17th, 2018 •
1,2,3,4, 2,4
1Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, 2Institute for Cell Engineering, School of Medicine, Johns Hopkins University, 3Institute for Nanobiotechnology, Johns Hopkins University, 4Department of Pathology, School of Medicine, Johns Hopkins University

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As células T antígeno-específicos são difíceis de caracterizar ou utilizar em terapias devido sua extremamente baixa frequência. Neste documento, nós fornecemos um protocolo para desenvolver uma partícula magnética que pode ligar para as células T antígeno-específicas para enriquecer estas células e então para expandi-los vários cem para caracterização e terapia.

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Engenharia edição 141 nanopartículas enriquecimento magnético artificiais células apresentadoras de antígeno células T imunoterapia câncer enriquecimento célula rara expansão de células T terapia adotiva de célula T Neoantigen immunoengineering bioengenharia
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