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Journal / Chemistry / 利用毛细管区电泳串联质谱技术进行大尺度自上而下蛋白质组学…
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Chemistry

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JoVE Journal Chemistry
利用毛细管区电泳串联质谱技术进行大尺度自上而下蛋白质组学
Molten-Salt Synthesis of Complex Metal Oxide Nanoparticles
Journal (Chemistry)
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Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
 
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利用毛细管区电泳串联质谱技术进行大尺度自上而下蛋白质组学

Article DOI: 10.3791/58644-v • 10:05 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 2,3, 1
1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine
* These authors contributed equally

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通过毛细管区电泳-电喷雾-串联质谱 (CZE-proteoforms), 描述了蛋白质样品中的分离、鉴定和表征的详细协议。该协议可用于简单蛋白样品中 proteoforms 的高分辨率表征和复杂蛋白质组样本中 proteoforms 的大尺度鉴定。

Tags

化学 问题 140 自上而下蛋白质组学 毛细管区电泳 电喷雾电离 串联质谱 proteoform,大肠杆菌
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