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JoVE Journal Chemistry
Storstilet Top-down Proteomics brug af kapillar Zone elektroforese Tandem massespektrometri
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Storstilet Top-down Proteomics brug af kapillar Zone elektroforese Tandem massespektrometri

Article DOI: 10.3791/58644-v • 10:05 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 2,3, 1
1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine
* These authors contributed equally

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En detaljeret protokol er beskrevet for adskillelse, identifikation og karakterisering af proteoforms i protein prøver ved hjælp af kapillar zone elektroforese-electrospray Ionisation-tandem massespektrometri (CZE-ESI-MS/MS). Protokollen kan bruges til høj opløsning karakterisering af proteoforms i enkle protein prøver og omfattende identifikation af proteoforms i komplekst proteom prøver.

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Kemi spørgsmål 140 Top-down proteomics kapillær zone elektroforese electrospray Ionisation tandem massespektrometri proteoform Escherichia coli
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