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Storskaliga Top-down proteomik med kapillära zonen elektrofores Tandem Mass Spectrometry
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Storskaliga Top-down proteomik med kapillära zonen elektrofores Tandem Mass Spectrometry

Article DOI: 10.3791/58644-v • 10:05 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 2,3, 1
1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine
* These authors contributed equally

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Ett detaljerat protokoll beskrivs för separation, identifiering och karakterisering av proteoforms i protein prover med kapillära zonen elektrofores-elektrospray jonisering-tandem mass spectrometry (CZE-ESI-MS/MS). Protokollet kan användas för högupplösta karakterisering av proteoforms i enkla protein prover och storskalig kartläggning av proteoforms i komplexa proteomet prover.

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Kemi fråga 140 Top-down proteomik kapillära zonen elektrofores elektrospray jonisering tandem mass spectrometry proteoform Escherichia coli
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