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Journal / Environment / Reproduceerbaar dsRNA Microinjection en…
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JoVE Journal Environment
Reproduceerbaar dsRNA Microinjection en Oviposition Bioassay in muggen en huis vliegt
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Reproduceerbaar dsRNA Microinjection en Oviposition Bioassay in muggen en huis vliegt

Article DOI: 10.3791/58650-v • 08:41 min • November 8th, 2018
November 8th, 2018 •
1, 2,3, 1, 1,2, 1
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment, Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute

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Dit protocol beschrijft een microinjection methodologie die we hebben gestandaardiseerd en gebruikt jarenlang voor bepaalde hoeveelheden van nucleïnezuren rechtstreeks naar de Hemolymfe van muggen en huis vliegt. Dit protocol resulteert in minimale injectie sterfte en laat dosis gecorreleerd metingen van vruchtbaarheid.

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