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JoVE Journal Genetics
Isolieren, Sequenzierung und Analyse von extrazellulären MicroRNAs aus humanen mesenchymalen Stammzellen
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Isolieren, Sequenzierung und Analyse von extrazellulären MicroRNAs aus humanen mesenchymalen Stammzellen

Article DOI: 10.3791/58655-v • 10:55 min • March 8th, 2019
March 8th, 2019 •
*1, *1, 1,2, 1, 1, 1,2
1The Interdisciplinary Nanoscience Centre, Aarhus University, 2Department of Molecular Biology and Genetics, Aarhus University
* These authors contributed equally

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Dieses Protokoll wird veranschaulicht, wie extrazelluläre MicroRNAs von Zellkulturmedien für kleine RNA Bibliotheksbau und Sequenzierung der nächsten Generation zu reinigen. Verschiedenen Qualitätskontrolle Checkpoints werden beschrieben, Leser zu verstehen, was beim Arbeiten mit niedrigen Eingabesamples wie ExRNAs zu erwarten.

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Genetik Ausgabe 145 mesenchymale Stammzellen extrazelluläre RNAs Exosomen extrazelluläre Vesikel Ribonucleproteins Micro-RNAs kleine RNAs Sequenzierung der nächsten Generation kleine RNA Bibliotheksbau
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