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JoVE Journal Biochemistry
Fluorescerende sølv farging av proteiner i polyakrylamid Gels
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Fluorescerende sølv farging av proteiner i polyakrylamid Gels

Article DOI: 10.3791/58669-v • 06:24 min • April 21st, 2019
April 21st, 2019 •
1, 1, 2, 1
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

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Her beskriver vi en detaljert protokoll beskriver en ny fluorescerende flekker teknikk for totalt proteinet oppdagelsen i polyakrylamid gels. Protokollen bruker en sølv ion-spesifikke fluorescens turn-on sonde, som oppdager Ag+-protein komplekser, og eliminerer visse begrensninger av tradisjonelle kromogent sølv flekker.

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Biokjemi problemet 146 sølv flekker fluorescerende gel flekker natrium dodecyl sulfat-polyakrylamid gel geleelektroforese (SDS-side) aggregasjon-indusert utslipp (AIE) sølv ion sensor fluorogenic oppdagelsen Proteomikk
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