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Biochemistry

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JoVE Journal Biochemistry
Fluorescerende sølv farvning af proteinerne i polyacrylamidgeler
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Fluorescerende sølv farvning af proteinerne i polyacrylamidgeler

Article DOI: 10.3791/58669-v • 06:24 min • April 21st, 2019
April 21st, 2019 •
1, 1, 2, 1
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

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Her beskriver vi en detaljeret protokol skitserer en ny fluorescerende farvning teknik til påvisning af total protein i polyacrylamidgeler. Protokollen udnytter en sølv ion-specifikke fluorescens turn-on sonde, som registrerer Ag+-proteinkomplekser, og fjerner visse begrænsninger af traditionelle kromogent sølv pletter.

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Biokemi spørgsmålet 146 sølv farvning fluorescerende gel farvning sodium dodecyl sulfat-polyacrylamid gelelektroforese (SDS-PAGE) sammenlægning-induceret emission (Bjarne) sølv ion sensor fluorogenic opdagelse proteomics
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