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Biochemistry

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JoVE Journal Biochemistry
Tinción fluorescente de plata de proteínas en geles de poliacrilamida
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Tinción fluorescente de plata de proteínas en geles de poliacrilamida

Article DOI: 10.3791/58669-v • 06:24 min • April 21st, 2019
April 21st, 2019 •
1, 1, 2, 1
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

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Aquí, describimos un protocolo detallado que un fluorescente nuevo coloración técnica para la detección de la proteína total en geles de poliacrilamida. El protocolo utiliza una sonda de encendido plata de iones específicos de fluorescencia que detecta Ag+-complejos de proteínas y elimina ciertas limitaciones de manchas plata cromogénicos tradicionales.

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Bioquímica número 146 plata tinción fluorescente gel electroforesis del gel de la sulfato-poliacrilamida de sodio dodecyl (SDS-PAGE) inducida por la agregación de emisión (AIE) sensor de iones de plata fluorógenos detección proteómica
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