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JoVE Journal Biochemistry
Fluorescerande silverfärgning av proteiner i polyakrylamidgeler
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Fluorescerande silverfärgning av proteiner i polyakrylamidgeler

Article DOI: 10.3791/58669-v • 06:24 min • April 21st, 2019
April 21st, 2019 •
1, 1, 2, 1
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

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Här beskriver vi ett detaljerade protokoll som beskriver en ny fluorescerande färgning teknik för totalprotein upptäckt i polyakrylamidgeler. Protokollet använder tredjeparts en silver ion-specifika fluorescens turn-on sond, som upptäcker Ag+-proteinkomplex, och eliminerar vissa begränsningar av traditionella kromogen silver fläckar.

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Biokemi fråga 146 silverfärgning fluorescerande gel färgning sodium dodecyl sulfate-polyakrylamid gelelektrofores (SDS-PAGE) aggregation-inducerad utsläpp (AIE) silverjoner sensor fluorogenic upptäckt proteomik
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