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Cancer Research

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A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Article DOI: 10.3791/58672-v • 10:44 min • August 9th, 2019
August 9th, 2019 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1, 3, 1,2
1Surgery Branch, National Cancer Institute, NIH, 2Center for Cell-Based Therapy, National Cancer Institute, NIH, 3Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH
* These authors contributed equally

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Summary
Automatic Translation

This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.

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Three-dimensional Thymic Culture System Murine Induced Pluripotent Stem Cells Tumor Antigen-specific Thymic Emigrants IPSC-derived Thymic Emigrants Naive-like T Cells Physiological Relevance In Vivo Differential Method CD8 Alpha-beta Antigen-specific T Cells Functional Phenotype Proliferation Remodiformation Tumor Suppression Doctor Rafiqul Islam Post-doc OP9/DLL1 Cells OP9 Media Trypsin Cell Strainer
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