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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Samtidige kvantificering af anti-vektor og Anti-transgen-specifikke CD8+ T celler Via MHC Tetramer farvning efter Vaccination med en Viral vektor
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Samtidige kvantificering af anti-vektor og Anti-transgen-specifikke CD8+ T celler Via MHC Tetramer farvning efter Vaccination med en Viral vektor

Article DOI: 10.3791/58680-v • 08:10 min • November 28th, 2018
November 28th, 2018 •
1, 1, 1, 1
1Division of Virology, Department of Hygiene, Microbiology, Social Medicine, Medical University of Innsbruck

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Vi præsenterer her, en protokol for ex vivo kvalitativ påvisning af antigen-specifikke CD8+ T celler. Analyse er muligt med enkelt celle udvisninger fra organer eller fra små mængder af blod. En bred vifte af undersøgelser kræver analyse af cytotoksiske T-celle svar (vaccination og cancer immunterapi undersøgelser).

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Immunologi og infektion sag 141 mus blod antigen-specifikke CTLs MHC jeg FACS tetramer fænotyper kvantificering immunisering
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