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Journal / Genetics / Kromatin Immunoprecipitation af Murine brunt…
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Genetics

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JoVE Journal Genetics
Kromatin Immunoprecipitation af Murine brunt fedtvæv
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Kromatin Immunoprecipitation af Murine brunt fedtvæv

Article DOI: 10.3791/58682-v • 07:50 min • November 21st, 2018
November 21st, 2018 •
1, 1, 2, 1
1Biochemistry Department, Boston University School of Medicine, 2Bioinformatic Hub, Boston University

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Her beskriver vi en protokol for effektiv kromatin immunoprecipitation (ChIP) efterfulgt af høj overførselshastighed DNA-sekventering (ChIP-seq) af brunt fedtvæv (BAT) isoleret fra en mus. Denne protokol er velegnet til både kortlægning Histon ændringer og undersøge genome-wide lokalisering af ikke-Histon proteiner af interesse in vivo.

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Genetik sag 141 kromatin immunoprecipitation brunt fedtvæv næste generation sequencing mus fedt
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