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Kromatin Immunoprecipitation av murina Brun fettvävnad
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Kromatin Immunoprecipitation av murina Brun fettvävnad

Article DOI: 10.3791/58682-v • 07:50 min • November 21st, 2018
November 21st, 2018 •
1, 1, 2, 1
1Biochemistry Department, Boston University School of Medicine, 2Bioinformatic Hub, Boston University

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Här beskriver vi ett protokoll för effektiv kromatin immunoprecipitation (ChIP) följt av hög genomströmning DNA-sekvensering (ChIP-seq) av Brun fettvävnad (BAT) isolerad från en mus. Detta protokoll är lämplig för både kartläggning Histon ändringar och utreda genome-wide lokalisering av icke-histonglykoprotein proteiner av intresse i vivo.

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Genetik fråga 141 kromatin immunoprecipitation brun fettväv nästa generations sekvensering mus fett
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