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Journal / Developmental Biology / RNA-baserte omprogrammering av menneskelig…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
RNA-baserte omprogrammering av menneskelig primære fibroblaster til indusert Pluripotent stamceller
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RNA-baserte omprogrammering av menneskelig primære fibroblaster til indusert Pluripotent stamceller

Article DOI: 10.3791/58687-v • 11:39 min • November 26th, 2018
November 26th, 2018 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

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Her beskriver vi en klinisk relevant, høy effektivitet, mater-fri metode for å omprogrammere menneskelig primære fibroblaster i indusert pluripotent stilk celler med endrede mRNAs koding omprogrammering faktorer og moden microRNA-367/302 etterligner. Også inkludert er metoder for å vurdere omprogrammering effektivitet, utvide klonal iPSC koloniene, og Bekreft uttrykk for pluripotency markøren TRA-1-60.

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Utviklingspsykologi biologi problemet 141 utførte pluripotent stamceller (iPSCs) reprogramming menneskelig modifiserte mRNAs klinisk relevante microRNAs (miRNAs) fibroblaster
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