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Journal / Neuroscience / Encellede RNA sekventering af Fluorescently…
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JoVE Journal Neuroscience
Encellede RNA sekventering af Fluorescently mærket mus neuroner ved hjælp af manuel sortering og dobbelt In Vitro transskription med absolut tæller sekventering (DIVA-Seq)
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Encellede RNA sekventering af Fluorescently mærket mus neuroner ved hjælp af manuel sortering og dobbelt In Vitro transskription med absolut tæller sekventering (DIVA-Seq)

Article DOI: 10.3791/58690-v • 07:49 min • October 26th, 2018
October 26th, 2018 •
1, 1
1Cold Spring Harbor Laboratory

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Denne protokol beskriver den manuelle sortering procedure for at isolere enkelt fluorescently mærket neuroner efterfulgt af in vitro- transskription-baserede mRNA forstærkning til høj-dybde encellede RNA sekvensering.

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Neurovidenskab spørgsmål 140 manuel sortering neuron encellede RNA sekventering DIVA-FF. in vitro transskription lineær forstærkning unikke molekylære id (UMI)
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