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Journal / Neuroscience / Eencellige RNA Sequencing van Fluorescently…
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Neuroscience

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JoVE Journal Neuroscience
Eencellige RNA Sequencing van Fluorescently geëtiketteerde muis neuronen met behulp van handmatig sorteren en dubbele In Vitro transcriptie met Absolute aantallen Sequencing (DIVA-Seq)
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Eencellige RNA Sequencing van Fluorescently geëtiketteerde muis neuronen met behulp van handmatig sorteren en dubbele In Vitro transcriptie met Absolute aantallen Sequencing (DIVA-Seq)

Article DOI: 10.3791/58690-v • 07:49 min • October 26th, 2018
October 26th, 2018 •
1, 1
1Cold Spring Harbor Laboratory

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Dit protocol beschrijft de procedure om te isoleren van één fluorescently geëtiketteerde neuronen gevolgd door in vitro transcriptie gebaseerde mRNA versterking voor hoge-diepte eencellige RNA sequencing sorteren handleiding.

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Neurowetenschappen kwestie 140 handmatig sorteren neuron eencellige RNA sequencing DIVA-Seq in vitro transcriptie lineaire versterking moleculaire eenduidig (UMI)
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