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Journal / Biology / Gocciolina basati su codici a barre singola cella …
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Biology

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JoVE Journal Biology
Gocciolina basati su codici a barre singola cella trascrittomica dei tessuti dei mammiferi adulti
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Gocciolina basati su codici a barre singola cella trascrittomica dei tessuti dei mammiferi adulti

Article DOI: 10.3791/58709-v • 10:12 min • January 10th, 2019
January 10th, 2019 •
*1,2,3, *2, 2, 2, 1,4, 2, 1,4, 1,2,3
1Hotchkiss Brain Institute, University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary
* These authors contributed equally

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Questo protocollo descrive le procedure generali e controlli di qualità necessari per la preparazione di cellule singole mammifera adulte sane per le preparazioni di RNA-Seq unicellulare gocciolina-based, alta velocità di trasmissione. Parametri di sequenziazione, leggi l'allineamento e l'analisi bioinformatica di cella singola a valle sono inoltre forniti.

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Biologia numero 143 unicellulare adulto trascrittoma RNA RNA-Seq FACS
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