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Journal / Biology / Transcriptómica de célula basada en código de…
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Biology

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JoVE Journal Biology
Transcriptómica de célula basada en código de barras gotita de tejidos mamíferos adultos
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Transcriptómica de célula basada en código de barras gotita de tejidos mamíferos adultos

Article DOI: 10.3791/58709-v • 10:12 min • January 10th, 2019
January 10th, 2019 •
*1,2,3, *2, 2, 2, 1,4, 2, 1,4, 1,2,3
1Hotchkiss Brain Institute, University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary
* These authors contributed equally

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Este protocolo describe el proceso general y control de calidad controles necesarios para la preparación de células sanas de mamíferas adultos solo para preparaciones de RNA-Seq de celulares solo basada en gota, alto rendimiento. Secuenciación parámetros, lea alineación y análisis Bioinformático unicelular aguas abajo también se proporcionan.

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Biología número 143 unicelular RNA-Seq adulto transcriptoma RNA FACS
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