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Undersøkelse av genetisk avhengigheter ved hjelp av CRISPR-Cas9-baserte konkurranse analyser
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Undersøkelse av genetisk avhengigheter ved hjelp av CRISPR-Cas9-baserte konkurranse analyser

Article DOI: 10.3791/58710-v • 11:05 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1, 2, 3, 1
1Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute, 2Blood Research Institute, Blood Center of Wisconsin, 3Department of Bioengineering, University of California, San Diego

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Dette manuskriptet beskriver en gruppert regelmessig Interspaced kort Palindromic gjentar (CRISPR) CRISPR-Cas9-basert metode for enkel og rask undersøkelse av rollen som flere kandidat gener i akutt myelogen leukemi AML celle spredning i parallell. Denne teknikken er skalerbar og kan brukes i andre kreftcelle linjer også.

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Kreftforskning problemet 143 AML CRISPR-Cas9 høy gjennomstrømming analysen konkurransedyktig vekst analysen DOT1L leukemi kreft
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