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Immunology and Infection

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JoVE Journal Immunology and Infection
Multiplex fluorescerende immunhistokemisk farvning, billedbehandling og analyse i histologiske prøver af lymfom
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Multiplex fluorescerende immunhistokemisk farvning, billedbehandling og analyse i histologiske prøver af lymfom

Article DOI: 10.3791/58711-v • 07:52 min • January 9th, 2019
January 9th, 2019 •
*1,2, *3, 3, 2, 2, 2, 4, 4, 2,3, 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
* These authors contributed equally

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Her beskriver vi en protokol for multiplex fluorescerende immunhistokemisk farvning og imaging for simultan lokalisering af flere kræft-associerede antigener i lymfom. Denne protokol kan udvides til en colocalization analyse af biomarkører inden for alle væv sektioner.

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Immunologi og infektion spørgsmålet 143 Multiplexed immunofluorescens multispektrale imaging kvantitativ analyse biomarkører lymfom digital patologi
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