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Immunology and Infection

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JoVE Journal Immunology and Infection
Coloração imuno-histoquímica fluorescente multiplexada, imagem e análise de amostras histológicas de linfoma
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Coloração imuno-histoquímica fluorescente multiplexada, imagem e análise de amostras histológicas de linfoma

Article DOI: 10.3791/58711-v • 07:52 min • January 9th, 2019
January 9th, 2019 •
*1,2, *3, 3, 2, 2, 2, 4, 4, 2,3, 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
* These authors contributed equally

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Aqui descrevemos um protocolo para multiplex immunohistochemical fluorescente de coloração e de imagem para localização simultânea de múltiplos antígenos associada a câncer de linfoma. Este protocolo pode ser estendido para a análise de colocalization de biomarcadores dentro de todas as secções de tecido.

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Imunologia e infecção edição 143 multiplexados imunofluorescência multispectral imaging análise quantitativa de biomarcadores linfoma patologia digital
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