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Immunology and Infection

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JoVE Journal Immunology and Infection
Multiplexade fluorescerande immunhistokemisk färgning, bildbehandling och analys i histologiska prover av lymfom
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Multiplexade fluorescerande immunhistokemisk färgning, bildbehandling och analys i histologiska prover av lymfom

Article DOI: 10.3791/58711-v • 07:52 min • January 9th, 2019
January 9th, 2019 •
*1,2, *3, 3, 2, 2, 2, 4, 4, 2,3, 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
* These authors contributed equally

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Här beskriver vi ett protokoll för multiplex fluorescerande immunhistokemisk färgning och tänkbar för samtidiga localizationen av flera cancer-associerade antigener i lymfom. Detta protokoll kan utvidgas till en colocalization analys av biomarkörer inom alla vävnadssnitt.

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Immunologi och infektion fråga 143 multiplexöverförde immunofluorescens Multispektral avbildning kvantitativ analys biomarkörer lymfom digital patologi
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