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Journal / Genetics / Avgörande 3'-Termini och sekvenser av begynnande…
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Genetics

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JoVE Journal Genetics
Avgörande 3'-Termini och sekvenser av begynnande enkelsträngat virala DNA-molekyler under HIV-1 omvänd Transkription i infekterade celler
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Journal (Genetics)
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
Journal (Genetics)
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
 
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Avgörande 3'-Termini och sekvenser av begynnande enkelsträngat virala DNA-molekyler under HIV-1 omvänd Transkription i infekterade celler

Article DOI: 10.3791/58715-v • 13:07 min • January 30th, 2019
January 30th, 2019 •
1, 1, 1
1Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London

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Här presenterar vi en djupsekvensering strategi som ger en objektiv bestämning av begynnande 3'-termini samt mutationsanalys profiler enkelsträngat DNA-molekyler. Huvudprogrammet är karakterisering av begynnande retrovirala kompletterande DNAs (cDNAs), de intermediärer som genereras under processen för retrovirala omvänd Transkription.

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Genetik fråga 143 3'-terminus kartläggning DNA polymerisation virusreplikation intermediärer HIV-1 omvänt avskrifter opartisk ssDNA ligatur adapter ligatur djupt sekvensering
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