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JoVE Journal Biochemistry
Analyse protéomique de polarisation des macrophages humains dans un environnement pauvre en oxygène
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Analyse protéomique de polarisation des macrophages humains dans un environnement pauvre en oxygène

Article DOI: 10.3791/58727-v • 10:15 min • January 7th, 2019
January 7th, 2019 •
1,2, 1,2, 1,2,3
1Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, 2Université Grenoble Alpes, 3Pôle Recherche, Centre Hospitalier Universitaire des Alpes

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Nous présentons un protocole visant à obtenir les signatures de protéomique des macrophages humains et cela s’applique à la détermination de l’impact d’un environnement pauvre en oxygène sur la polarisation de macrophages.

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Numéro 143 Macrophage polarisation biochimie protéome LC-MS/MS hypoxie hors-gel fractionnement
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