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JoVE Journal
Biology

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Kvantitative Immunoblotting af cellelinjer som Standard til at validere immunofluorescens for kvantificering biomarkør proteiner i rutinemæssige vævsprøver
 
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Kvantitative Immunoblotting af cellelinjer som Standard til at validere immunofluorescens for kvantificering biomarkør proteiner i rutinemæssige vævsprøver

Article DOI: 10.3791/58735-v 09:58 min January 7th, 2019
January 7th, 2019

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Summary

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Vi beskriver brugen af kvantitative immunoblotting at validere immunofluorescens histologi kombineret med billedanalyse som et middel til at kvantificere en protein af interesse i formalin-fast, paraffin-embedded (FFPE) vævsprøver. Vores resultater viser nytten af immunofluorescens histologi for fastslå det relative antal biomarkør proteiner i rutinemæssig biopsi prøver.

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Biologi spørgsmålet 143 immunofluorescens formalin-fast paraffin-embedded kvantitative immunblot vestlige skamplet patologi protein biomarkør histologi immunohistology
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