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Journal
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Neuroscience
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神经科学中透射电镜切片采样的一种无偏方法
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
An Unbiased Approach of Sampling TEM Sections in Neuroscience
Please note that all translations are automatically generated.
Click here for the English version.
神经科学中透射电镜切片采样的一种无偏方法
DOI:
10.3791/58745-v
•
10:56 min
•
April 13, 2019
•
Stefan Wernitznig
,
Florian Reichmann
,
Mariella Sele
,
Christoph Birkl
,
Johannes Haybäck
5
,
Florian Kleinegger
,
Anna Birkl-Töglhofer
,
Stefanie Krassnig
,
Christina Wodlej
,
Peter Holzer
,
Daniel Kummer
,
Elisabeth Bock
,
Gerd Leitinger
1
Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center
,
Medical University of Graz
,
2
Department of Pharmacology, Otto Loewi Research Center
,
Medical University of Graz
,
3
Division of General Neurology, Department of Neurology
,
Medical University of Graz
,
4
Institute of Pathology
,
Medical University of Graz
,
5
Department of Pathology, Medical Faculty
,
Otto von Guericke University Magdeburg
Chapters
00:04
Title
01:02
TEM Preparation Embedding, Ultra-Thin Sectioning and Staining
02:05
Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages
05:25
Analyze the TEM images with ImageJ to Document Ultrastructural Features
07:10
Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph
09:19
Results: Unbiased Approach of Sampling TEM Sections
10:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们介绍了一种新的脑组织电子显微镜研究工作流程。该方法允许用户以无偏见的方式检查神经元特征。对于元素分析, 我们还提供了一个脚本, 该脚本自动化了随机采样的大部分工作流。
Tags
– Unbiased Sampling- Electron Microscopy- Neuroscience- Quantification- Numerical Density- Synapses- Neural Network- Sample Preparation- Ultramicrotome- Serial Sections- Slot Grid- Counterstaining- Transmission Electron Microscope (TEM)- TEM Serial Section Software- Region Of Interest (ROI)- Random Point Sampling
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