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Efeito de proteínas fluorescentes na fusão parceiros usando Polyglutamine ensaios de toxicidade em levedura
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Efeito de proteínas fluorescentes na fusão parceiros usando Polyglutamine ensaios de toxicidade em levedura

Article DOI: 10.3791/58748-v • 09:23 min • November 28th, 2018
November 28th, 2018 •
1, 2, 1, 1,2, 1
1Department of Anatomy and Cell Biology, University of Western Ontario, 2Department of Pathology and Laboratory Medicine, University of Western Ontario

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Este artigo descreve os protocolos para avaliar o efeito de proteínas fluorescentes na agregação e toxicidade de misfolded polyglutamine expansão para a avaliação rápida de uma proteína fluorescente recentemente descaracterizada no contexto dos repórteres fluorescentes.

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Biologia edição 141 proteínas fluorescentes toxicidade polyglutamine ensaios de crescimento de levedura agregação proteína verde fluorescente microscopia fluorescente
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