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Journal / Immunology and Infection / Analyse van Shear Flow-geïnduceerde migratie van…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse van Shear Flow-geïnduceerde migratie van RattenUitrustingen Marginal Zone B cellen In Vitro
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
Journal (Immunology and Infection)
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Journal (Immunology and Infection)
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
 
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Analyse van Shear Flow-geïnduceerde migratie van RattenUitrustingen Marginal Zone B cellen In Vitro

Article DOI: 10.3791/58759-v • 08:31 min • November 26th, 2018
November 26th, 2018 •
1, 1, 1, 1, 1
1Institute of Biochemistry and Cell Biology, OVGU University of Magdeburg

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Marginaal zone B cellen (MZBs) reageren op de kracht van shear stroom door heroriëntering op hun migratiepad van de stroom. Dit protocol laat zien hoe om te registreren en analyseren van het migratie met behulp van een fluidics eenheid, pomp, Microscoop imaging systeem en vrije software.

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Immunologie en infecties probleem 141 B-cellen marginale zone migratie schuintrekken stroom integrins adhesie intercellulaire adhesie molecuul-1 (ICAM-1) vasculaire cel adhesie molecuul-1 (VCAM-1)
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