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Journal / Genetics / CRISPR-medieret genom-redigering af menneskelige…
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Genetics

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JoVE Journal Genetics
CRISPR-medieret genom-redigering af menneskelige svampe patogen Candida albicans
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CRISPR-medieret genom-redigering af menneskelige svampe patogen Candida albicans

Article DOI: 10.3791/58764-v • 09:56 min • November 14th, 2018
November 14th, 2018 •
*1, *1, 2, 1
1Department of Biology, Ball State University, 2Whitehead Institute for Biomedical Research
* These authors contributed equally

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Effektiv genom engineering af Candida albicans er afgørende for forståelsen af patogenesen og udvikling af lægemidler. Her, beskrev vi en protokol for at hurtigt og præcist redigere C. albicans genom ved hjælp af CRISPR. Protokollen giver mulighed for efterforskerne at indføre en lang række genetiske modifikationer herunder punktmutationer, indsætninger og sletninger.

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Genetik sag 141 CRISPR Candida albicans genom-redigering transformation Cas9 patogener svampe
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