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Journal / Neuroscience / To-fotonet Imaging Microglial prosesser…
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Neuroscience

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JoVE Journal Neuroscience
To-fotonet Imaging Microglial prosesser attraksjon mot ATP eller Serotonin i akutt hjernen skiver
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To-fotonet Imaging Microglial prosesser attraksjon mot ATP eller Serotonin i akutt hjernen skiver

Article DOI: 10.3791/58788-v • 07:27 min • January 31st, 2019
January 31st, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, *1,2,3, *1,2,3
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France
* These authors contributed equally

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Microglia, bosatt immunceller av hjernen, svare raskt morfologiske endringer til endringer i deres miljø. Denne protokollen beskriver hvordan du bruker to-fotonet mikroskopi for å studere tiltrekningen av microglial prosesser mot serotonin eller ATP i akutt hjernen skiver av mus.

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Nevrovitenskap problemet 143 Microglia flere Foton mikroskopi akutt hjernen skiver chemoattraction motilitet morfologi serotonin ATP live bildebehandling tidsinnstilt bildebehandling
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