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Journal / Neuroscience / Twee-foton beeldvorming van Microglial processen…
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Neuroscience

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JoVE Journal Neuroscience
Twee-foton beeldvorming van Microglial processen attractie naar ATP of serotonine in Acute hersenen plakjes
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Twee-foton beeldvorming van Microglial processen attractie naar ATP of serotonine in Acute hersenen plakjes

Article DOI: 10.3791/58788-v • 07:27 min • January 31st, 2019
January 31st, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, *1,2,3, *1,2,3
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France
* These authors contributed equally

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Microglia, de resident immuuncellen van de hersenen, reageren snel met de morfologische veranderingen voor wijzigingen van hun omgeving. Dit protocol wordt beschreven hoe u twee-foton microscopie te bestuderen van de aantrekkingskracht van microglial processen naar serotonine of ATP in acute hersenen plakjes van muizen.

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Neurowetenschappen kwestie 143 Microglia multi foton microscopie acute hersenen segmenten chemoattraction beweeglijkheid morfologie serotonine ATP live beeldvorming time-lapse imaging
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