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JoVE Journal Genetics
Generation af kræft celle kloner til at visualisere Telomeric Repeat-holdige RNA TERRA udtrykt fra en enkelt Telomer i levende celler
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Generation af kræft celle kloner til at visualisere Telomeric Repeat-holdige RNA TERRA udtrykt fra en enkelt Telomer i levende celler

Article DOI: 10.3791/58790-v • 09:13 min • January 17th, 2019
January 17th, 2019 •
1, 1, 1,2, 1
1Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, 2Department of Life Sciences, University of Trieste

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Vi præsenterer her, en protokol for at generere kræft celle kloner indeholdende en MS2 sekvens tag på en enkelt subtelomere. Denne tilgang, lid MS2-normal god landbrugspraksis system, giver mulighed for visualisering af de endogene afskrifter af telomeric repeat-holdige RNA (TERRA) udtrykt fra en enkelt Telomer i levende celler.

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Genetik spørgsmålet 143 lange noncoding RNA TERRA telomere kræft CRISPR/Cas9 MS2-normal god landbrugspraksis live-celle billeddannelse.
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