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Journal / Genetics / Generering av kreft cellen kloner å visualisere…
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JoVE Journal Genetics
Generering av kreft cellen kloner å visualisere Telomeric gjenta inneholder RNA TERRA uttrykt fra en enkelt Telomere i levende celler
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Generering av kreft cellen kloner å visualisere Telomeric gjenta inneholder RNA TERRA uttrykt fra en enkelt Telomere i levende celler

Article DOI: 10.3791/58790-v • 09:13 min • January 17th, 2019
January 17th, 2019 •
1, 1, 1,2, 1
1Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, 2Department of Life Sciences, University of Trieste

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Her presenterer vi en protokoll for å generere kreft cellen kloner en MS2 sekvens kode på en enkelt subtelomere. Denne tilnærmingen, avhengig av MS2-GFP systemet, gjør visualisering av endogene transkripsjoner av telomeric gjenta inneholder RNA (TERRA) uttrykt fra en enkelt telomere i levende celler.

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Genetikk problemet 143 lang noncoding RNA TERRA telomere kreft CRISPR/Cas9 MS2-GFP live-celle bildebehandling.
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