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Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

Article DOI: 10.3791/58791-v • 06:01 min • February 2nd, 2019
February 2nd, 2019 •
1, 2, 2, 3, 2
1Cell & Molecular Biology Program, University of Arkansas, 2Department of Biomedical Engineering, University of Arkansas, 3Ralph E. Martin Department of Biomedical Engineering, University of Arkansas

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The goal of this protocol is production of whole extracellular matrix fibers targeted for wound repair which are suitable for preclinical evaluation as part of a regenerative scaffold implant. These fibers are produced by the culture of fibroblasts on hollow fiber membranes and extracted by dissolution of the membranes.

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Extracellular Matrix Fibers Hollow Fiber Membrane Cell Culture Filaments Bulk Extracellular Matrix Cell Matrix Interactions Biomaterial Wound Repair Synthetic Fibers Foreign Body Response Textile Fabrication Techniques Woven Implantables Autoclave Bovine Plasma Fibronectin Fibroblast Cells Petri Dish Fibroblast Culture Medium L-ascorbic Acid L-ascorbic Acid 2 Phosphate Transformin
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