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JoVE Journal Biochemistry
Sondering struktur og dynamik af Nucleosomes ved hjælp af Atomic Force mikroskopi Imaging
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Sondering struktur og dynamik af Nucleosomes ved hjælp af Atomic Force mikroskopi Imaging

Article DOI: 10.3791/58820-v • 09:52 min • January 31st, 2019
January 31st, 2019 •
1, 1, 1, 1
1Department of Pharmaceutical Sciences, University of Nebraska Medical Center

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Vi præsenterer her, en protokol til at karakterisere nucleosome partikler på enkelt-molekyle niveau ved hjælp af statisk og time-lapse atomic force mikroskopi (AFM) imaging teknikker. Den beskrevne overflade functionalization metode giver mulighed for opsamling af strukturen og dynamikken i nucleosomes i høj opløsning på nanoplan.

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Biokemi spørgsmålet 143 AFM time-lapse nucleosome kromatin dynamics struktur histoner enkelt - molekyle DNA Epigenetik imaging
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