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Journal / Biochemistry / Användning av rekombinanta fusionsproteinerna i…
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Biochemistry
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JoVE Journal Biochemistry
Användning av rekombinanta fusionsproteinerna i en fluorescerande proteas Assay plattform och deras i-gel utvinningsarbete
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Användning av rekombinanta fusionsproteinerna i en fluorescerande proteas Assay plattform och deras i-gel utvinningsarbete

Article DOI: 10.3791/58824-v • 19:23 min • January 16th, 2019
January 16th, 2019 •
*1,2, *1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, 2Biotechnology Research Department, Gedeon Richter Plc
* These authors contributed equally

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Här presenterar vi de detaljerade anvisningar av en nyligen utvecklad proteas assay plattform använder N-terminala hexahistidine/maltos-bindande protein och fluorescerande protein-smält rekombinant substrat kopplad till ytan av nickel-nitrilotriacetic sura magnetiska agaros pärlor. Efterföljande i-gel analys av assay proverna åtskilda av sodium dodecyl sulfate-polyakrylamid gelelektrofores presenteras också.

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Biokemi fråga 143 rekombinant fusion protein substrat proteas assay Ni-NTA magnetiska agaros pärlor fluorescerande protein i-gel utvinningsarbete humant immunbristvirus typ 1 proteas
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